VitroGel Glioblastoma (GBM) EMT Kit, XF | Basement Membrane Extract (BME)-free Database

Commercial source

TheWell Bioscience

URL

https://www.thewellbio.com/product/vitrogel-glioblastoma-emt-kit-xf/

Product details

Origin

Synthetic

Type of material

Polysaccharide-based hydrogel

Type of gelation/Coating

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca²⁺ or Na⁺)

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

U-87MG ATCC (RRID:CVCL_0022)

Primary cells

Nervous system

Tumour

Glioblastoma (GBM) cells

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

Stiffness = 100−1000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Van der Waals interactions

Application specifications

Cell retrieval

Yes

VitroGel Organoid Recovery Solution (enzyme-free)

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – VitroGel Glioblastoma (GBM) EMT Kit

Downstream analysis

Protocol – VitroGel Organoid Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit

Keywords

Epithelial-mesenchymal transition (EMT) assay, High-throughput screening