Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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VitroGel MMP High Concentration

TheWell Bioscience
https://www.thewellbio.com/product/vitrogel-mmp/
Synthetic

Polysaccharide-based hydrogel

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)

2D & 3D
Other
BL-5 (RRID:CVCL_M563)
HeLa (RRID:CVCL_0030)
LRM55
MCF-10A (RRID:CVCL_0598)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
MDCK (RRID:CVCL_0422)
OVCA429 (RRID:CVCL_3936)
OVCAR-3 (RRID:CVCL_0465)
PANC-1 (RRID:CVCL_0480)
PC-3 (RRID:CVCL_0035)
SNB-75 (RRID:CVCL_1706)
T-47D (RRID:CVCL_0553)
U-373MG ATCC (RRID:CVCL_2219)
U-87MG ATCC (RRID:CVCL_0022)
WM239A (RRID:CVCL_6795)
Human umbilical artery smooth muscle cells (HUASMCs) & Human umbilical vein endothelial cells (HUVECs)
Yes
Yes
Yes
Specified

G’ = 10−4000 Pa

Yes

Unspecified

Yes

VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)

No
Yes

Protocol – VitroGel MMP High Concentration

Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit
Protocol – Cell invasion assay

Invasion assay, MMP

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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