Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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PeptiGel Gamma 4

Cell Guidance Systems
https://www.cellgs.com/products/peptigelandreg-gamma-4and8482.html
Synthetic

Self-assembled peptide hydrogel (positively charged)

By adding cell culture media. The ionic salts present in the media screen the charges present on the peptide fibres which causes them to aggregate and induce gelation at room temperature.

2D & 3D
Other
Unspecified
Yes
Yes
Yes
Specified

Stiffness = 0.175−0.350 kPa

Yes

Unspecified

Unspecified
Unspecified
Yes

Protocol – 2D cell culture
Protocol – 3D cell culture

Protocol – PicoGreen cell viability assay for PeptiGel cultures
Protocol – Sample preparation for protein extraction from PeptiGel cultures
Protocol – Sample preparation for RNA extraction from PeptiGel cultures
Protocol – How to process PeptiGel cultures for cell staining

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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