Cell Guidance Systems
Synthetic
Self-assembled peptide hydrogel (positively charged)
By adding cell culture media. The ionic salts present in the media screen the charges present on the peptide fibres which causes them to aggregate and induce gelation at room temperature.
2D & 3D
Organoids, Spheroids
Unspecified
Yes
Yes
Yes
Specified
Stiffness = 0.175−0.350 kPa
Yes
Unspecified
Unspecified
Unspecified
Unspecified
Unspecified
Unspecified
Yes
Protocol – PicoGreen cell viability assay for PeptiGel cultures
Protocol – Sample preparation for protein extraction from PeptiGel cultures
Protocol – Sample preparation for RNA extraction from PeptiGel cultures
Protocol – How to process PeptiGel cultures for cell staining
Protocol – How to extract organoids from PeptiGel cultures
Collagen, RGD