TrueGel3D Hydrogel Kit 5 (TRUE5) | Basement Membrane Extract (BME)-free Database

Commercial source

Merck, Sigma-Aldrich

URL

https://www.sigmaaldrich.com/NL/en/product/sigma/true5

Product details

Origin

Synthetic

Type of material

Polyvinyl alcohol (PVA)-based hydrogel

Type of gelation/Coating

The (thiol-reactive) maleimide-functionalised PVA polymer is crosslinked by the thiol-functionalised polyethylene glycol (PEG) non-degradable crosslinker.

Type of in vitro culture

Type of application

Spheroids

Cell type

Cell lines

MDCK (RRID:CVCL_0422)
NIH 3T3 (RRID:CVCL_0594)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Unspecified

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. Peptides with a N- or C-terminal cysteine can be covalently linked to the hydrogel.

Application specifications

Cell retrieval

Dome formation

Unspecified

Imaging

Autofluorescent

Unspecified

Transparent

Yes

Instructions

Product instructions

Downstream analysis

Protocol – How to customize the TrueGel3D™ hydrogel