3-D Life Dextran-CD Hydrogel FG + RGD Peptide | Basement Membrane Extract (BME)-free Database

Commercial source

Cellendes

URL

https://www.cellendes.com/products/kits/product/kfg91-1, https://www.cellendes.com/products/kits/product/p09-p-001

Product details

Origin

Natural, Synthetic

Type of material

Dextran-based hydrogel

Type of gelation/Coating

Dextran is covalently crosslinked by CD-Link via the Michael addition reaction. CD-Link is a metalloproteinase (MMP)-cleavable peptide conjugated to polyethylene glycol (PEG).

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

C2C12 (RRID:CVCL_0188)
MCF-7 (RRID:CVCL_0031)
NIH 3T3 (RRID:CVCL_0594)
OCI-AML-3 (RRID:CVCL_1844)

Primary cells

Blood

Dendritic cells (DCs)

Human umbilical vein endothelial cells (HUVECs)

Stem cells

Adult stem cells

Bone marrow-derived mesenchymal stem/stromal cells (BMSCs)

Co-culture

C2C12 (CVCL_0188) & NIH 3T3 (CVCL_0594)
Dermal fibroblasts & MCF-7 (RRID:CVCL_0031)
Human umbilical vein endothelial cells (HUVECs) & NIH 3T3 (CVCL_0594)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 40−4000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. 3-D Life peptides or custom-made peptides with thiol groups can be covalently attached to the hydrogel.

Application specifications

Cell retrieval

Yes

Enzymatic digestion by 3-D Life Dextranase.

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – Preparation of 3-D Life Fast Gelling Hydrogels

User guide – 3-D Life Hydrogels

Downstream analysis

Protocol – Cell recovery

Keywords

Chemotaxis assay, High-throughput screening, RGD