Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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3-D Life Dextran-CD Hydrogel SG + RGD Peptide

Cellendes
https://www.cellendes.com/products/kits/product/kg93-1, https://www.cellendes.com/products/kits/product/p09-p-001
Natural, Synthetic

Dextran-based hydrogel

Dextran is covalently crosslinked by CD-Link via the Michael addition reaction. CD-Link is a metalloproteinase (MMP)-cleavable peptide conjugated to polyethylene glycol (PEG).

3D
Spheroids, Other
HaCaT (RRID:CVCL_0038)
HCT 116 (RRID:CVCL_0291)
Hep-G2 (RRID:CVCL_0027)
HL-60 (RRID:CVCL_0002)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
NCI-H838 (RRID:CVCL_1594)
NIH 3T3 (RRID:CVCL_0594)
PANC-1 (RRID:CVCL_0480)
U-87MG ATCC (RRID:CVCL_0022)
U-937 (RRID:CVCL_0007)
Breast cancer organoids & CAR T cells & MDA-MB-231 (CVCL_0062) & ROR1− fibroblasts & T cells
Dermal fibroblasts & HCT 116 (CVCL_0291) & HL-60 (CVCL_0002) & Human umbilical vein endothelial cells (HUVECs) & NCI-H838 (CVCL_1594) & U-937 (CVCL_0007)
Dermal fibroblasts & MCF-7 (RRID:CVCL_0031)
HaCaT (CVCL_0038) & HL-60 (CVCL_0002) & MCF-7 (CVCL_0031) & NCI-H838 (CVCL_1594) & U-937 (CVCL_0007)
Melanocytes & Peripheral blood mononuclear cells (PBMCs) & T cells
Yes
Yes
Yes
Specified

G’ = 20−10,000 Pa

Yes

Thiol-mediated conjugation. 3-D Life peptides or custom-made peptides with thiol groups can be covalently attached to the hydrogel.

Yes

Enzymatic digestion by 3-D Life Dextranase.

Yes
No
Yes

Protocol – Preparation of 3-D Life Slow Gelling Hydrogels

User guide – 3-D Life Hydrogels

Protocol – Cell recovery

Cell extravasation assay, Chemotaxis assay, Cytokine release assay, High-throughput screening, Organ-on-a-chip model, RGD, Tumour infiltration assay

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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