3-D Life Dextran-PEG Hydrogel FG + RGD Peptide | Basement Membrane Extract (BME)-free Database

Commercial source

Cellendes

URL

https://www.cellendes.com/products/kits/product/kfg90-1, https://www.cellendes.com/products/kits/product/p09-p-001

Product details

Origin

Natural, Synthetic

Type of material

Dextran-based hydrogel

Type of gelation/Coating

Dextran is covalently crosslinked by PEG-Link (thiol-modified polyethylene glycol) via the Michael addition reaction.

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

HCT 116 (RRID:CVCL_0291)
HKC-8 (RRID:CVCL_Y910)
MCF-10A (RRID:CVCL_0598)
NIH 3T3 (RRID:CVCL_0594)

Primary cells

Blood

Dendritic cells (DCs)

Human umbilical vein endothelial cells (HUVECs)

Cartilage

Chondrocytes

Kidney

Renal fibroblasts

Co-culture

HKC-8 (RRID:CVCL_Y910) & Renal fibroblasts
Human umbilical vein endothelial cells (HUVECs) & NIH 3T3 (CVCL_0594)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 40−5000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. 3-D Life peptides or custom-made peptides with thiol groups can be covalently attached to the hydrogel.

Application specifications

Cell retrieval

Yes

Enzymatic digestion by 3-D Life Dextranase.

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – Preparation of 3-D Life Fast Gelling Hydrogels

User guide – 3-D Life Hydrogels

Downstream analysis

Protocol – Cell recovery

Keywords

High-throughput screening, RGD