3-D Life Dextran-PEG Hydrogel SG | Basement Membrane Extract (BME)-free Database

Commercial source

Cellendes

URL

https://www.cellendes.com/products/kits/product/kg92-1

Product details

Origin

Natural, Synthetic

Type of material

Dextran-based hydrogel

Type of gelation/Coating

Dextran is covalently crosslinked by PEG-Link (thiol-modified polyethylene glycol) via the Michael addition reaction.

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

Jurkat (RRID:CVCL_0065)
MCF-7 (RRID:CVCL_0031)

Primary cells

Blood

Tumour

Chronic lymphocytic leukaemia (CLL) cells

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 100−10,000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. 3-D Life peptides or custom-made peptides with thiol groups can be covalently attached to the hydrogel.

Application specifications

Cell retrieval

Yes

Enzymatic digestion by 3-D Life Dextranase.

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – Preparation of 3-D Life Slow Gelling Hydrogels

User guide – 3-D Life Hydrogels

Downstream analysis

Protocol – Cell recovery

Keywords

High-throughput screening, Organ-on-a-chip model