3-D Life PVA-CD Hydrogel FG + RGD Peptide | Basement Membrane Extract (BME)-free Database

Commercial source

Cellendes

URL

https://www.cellendes.com/products/kits/product/kfg81-1, https://www.cellendes.com/products/kits/product/p09-p-001

Product details

Origin

Synthetic

Type of material

Hydrogel based on polyvinyl alcohol (PVA).

Type of gelation/Coating

PVA is covalently crosslinked by CD-Link via the Michael addition reaction. CD-Link is a metalloproteinase (MMP)-cleavable peptide conjugated to polyethylene glycol (PEG).

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

NIH 3T3 (RRID:CVCL_0594)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 40−2000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. 3-D Life peptides or custom-made peptides with thiol groups can be covalently attached to the hydrogel.

Application specifications

Cell retrieval

Yes

Enzymatic digestion by trypsin.

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – Preparation of 3-D Life Fast Gelling Hydrogels

User guide – 3-D Life Hydrogels

Keywords

High-throughput screening, Invasion assay, Migration assay, RGD