3-D Life ToGro Hydrogel | Basement Membrane Extract (BME)-free Database

Commercial source

Cellendes

URL

https://www.cellendes.com/products/kits/product/kg94-1

Product details

Origin

Natural, Synthetic

Type of material

Dextran-based hydrogel containing a RGD peptide.

Type of gelation/Coating

Dextran is covalently crosslinked by CD-Link via the Michael addition reaction. CD-Link is a metalloproteinase (MMP)-cleavable peptide conjugated to polyethylene glycol (PEG).

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

NIH 3T3 (RRID:CVCL_0594)

Stem cells

Adult stem cells

Enteric neural stem cells

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 400 Pa

Possibility to conjugate biomolecules

Application specifications

Cell retrieval

Yes

Enzymatic digestion by 3-D Life Dextranase.

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – 3-D Cell Culture with 3-D Life ToGro Hydrogel

User guide – 3-D Life Hydrogels

Downstream analysis

Protocol – Cell recovery

Keywords

Cell extravasation assay, Chemotaxis assay, Cytokine release assay, High-throughput screening, Invasion assay, Migration assay, RGD, Tumour infiltration assay