Biolaminin 521 LN (LN521) | Basement Membrane Extract (BME)-free Database

Commercial source

BioLamina

URL

https://biolamina.com/products/laminin-ln-521-stem-cell-matrix/

Product details

Origin

Natural

Type of material

Human recombinant laminin-521 protein (0.1 mg mL⁻¹; full-length)

Type of gelation/Coating

Coating

Type of in vitro culture

Type of application

Embryonic stem cells (ESCs), Induced pluripotent stem cells (iPSCs)

Cell type

Cell lines

U-251MG (RRID:CVCL_0021)

Primary cells

Blood

Endothelial cells

Megakaryocytes

Bone

Unspecified

Breast

Mammary epithelial cells

Cartilage

Unspecified

Eye

Corneal endothelial cells

Photoreceptor cells

Retinal endothelial cells

Retinal pigment epithelial (RPE) cells

Gallbladder

Cholangiocytes

Heart

Cardiac progenitor cells

Cardiomyocytes

Intestine

Intestinal epithelial cells

Kidney

Glomerular endothelial cells (GECs)

Podocytes

Tubular epithelial cells

Liver

Hepatocytes

Lung

Bronchial epithelial cells

Muscle

Myocytes

Myogenic progenitor cells

Nervous system

Glial cells

Neurons

Unspecified

Pancreas

Beta cells

Reproductive system (female)

Unspecified

Skin

Dermal fibroblasts

Keratinocytes

Melanoblasts

Spleen

Mesothelial cells

Splenic endothelial cells

Stem cells

Adult stem cells

Bone marrow-derived mesenchymal stem/stromal cells (BMSCs)

Haematopoietic stem cells (HSCs)

Hair follicle stem cells (HFSCs)

Mesenchymal stem/stromal cells (MSCs)

Myosatellite cells

Neural stem cells (NSCs)

Embryonic stem cells (ESCs)

General

HS181 (RRID:CVCL_C229)

HS980 (RRID:CVCL_2Z88)

Induced pluripotent stem cells (iPSCs)

General

iPSC3 (RRID:CVCL_D088)

Pluripotent stem cells (PSCs)

General

Pluripotent stem cell (PSC)-derived cells

Embryonic stem cell (ESC)-derived cells

Astrocytes

Endothelial progenitor cells (EPCs)

Hepatocytes

Retinal pigment epithelial (RPE) cells

Induced pluripotent stem cell (iPSC)-derived cells

Astrocytes

Cardiomyocytes

Megakaryocytes

Pluripotent stem cell (PSC)-derived cells

Cardiomyocytes

Hepatocytes

Neural crest cells

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Unspecified

Possibility to conjugate biomolecules

Please note that recombinant laminins can be used to (bio)functionalise other products/hydrogels with to improve cellular functionality (e.g., cell adhesion, survival/proliferation, migration, differentiation and/or other cell type-specific responses).

Application specifications

Support maintenance pluripotency (PSCs)

Yes

Reference

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Instructions for use
Protocol – Culturing ESCs and iPSCs on Biolaminin 521

Downstream analysis

Protocol – Embryoid body formation from PSCs cultured on Biolaminin 521
Protocol – Neural crest differentiation from PSCs on Biolaminin 521