HyStem | Basement Membrane Extract (BME)-free Database

Commercial source

Advanced BioMatrix

URL

https://advancedbiomatrix.com/hystem2.0-kits.html

Product details

Origin

Synthetic

Type of material

Thiol-modified hyaluronic acid (Glycosil) hydrogel (10 mg mL⁻¹)

Type of gelation/Coating

The thiol groups of the thiol-modified hyaluronic acid react with the acrylate groups present in the polyethylene glycol diacrylate (PEGDA) crosslinker (Extralink), resulting in a hydrogel.

Type of in vitro culture

2D & 3D

Type of application

Embryonic stem cells (ESCs)

Cell type

Cell lines

A-549 (RRID:CVCL_0023)

Primary cells

Heart

Cardiomyocytes

Liver

Hepatic progenitor cells

Nervous system

Neural progenitor cells (NPCs)

Pancreas

Unspecified

Stem cells

Adult stem cells

Adipose-derived stem/stromal cells (ASCs)

Bone marrow-derived mesenchymal stem/stromal cells (BMSCs)

Haematopoietic stem cells (HSCs)

Hepatic stem cells

Mesenchymal stem/stromal cells (MSCs)

Embryonic stem cells (ESCs)

General

HUES 7 (RRID:CVCL_B205)

WA09 (RRID:CVCL_9773)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 110 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Michael addition reaction. Peptides with a N-terminal cysteine can be covalently linked to the hydrogel.

Application specifications

Support maintenance pluripotency (PSCs)

Unspecified

Reference

Cell retrieval

Yes

Enzymatic degradation (Collagenase/Hyaluronidase)

Imaging

Autofluorescent

Unspecified

Transparent

Yes

Instructions

Product instructions

Instructions for use
Protocol – 3D encapsulation using tissue culture inserts
Protocol – 3D encapsulation using 96-well plates

Downstream analysis

Protocol – Enzyme digestion of hydrogels for recovery of encapsulated cells