HyStem-C

Advanced BioMatrix
https://advancedbiomatrix.com/hystem-c3.html
Natural, Synthetic

Thiol-modified hyaluronic acid (Glycosil) and thiol-modified gelatin (Gelin-S) hydrogel (10 mg mL−1)

The thiol groups of the thiol-modified hyaluronic acid and thiol-modified gelatin react with the acrylate groups present in the polyethylene glycol diacrylate (PEGDA) crosslinker (Extralink), resulting in a hydrogel.

2D & 3D
Embryonic stem cells (ESCs), Spheroids, Other
A-549 (RRID:CVCL_0023)
T-47D (RRID:CVCL_0553)
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) & Vocal fold fibroblasts (VFF)
Yes
No
Yes
Specified

G’ = 159 Pa

Yes

Michael addition reaction. Peptides with a N-terminal cysteine can be covalently linked to the hydrogel.

Unspecified
Yes

Enzymatic degradation (Collagenase/Hyaluronidase)

Unspecified
Unspecified
Yes

Instructions for use
Protocol – 2D culture
Protocol – 3D encapsulation using tissue culture inserts
Protocol – 3D encapsulation using 96-well plates

Protocol – Enzyme digestion of hydrogels for recovery of encapsulated cells
Protocol – Trypsin cell recovery from the surface of hydrogels

Organ-on-a-chip model