Advanced BioMatrix
Natural, Synthetic
Thiol-modified hyaluronic acid, thiol-modified heparin (Heprasil) and thiol-modified gelatin (Gelin-S) hydrogel (10 mg mL−1)
The thiol groups of the thiol-modified hyaluronic acid, heparin and gelatin react with the acrylate groups present in the polyethylene glycol diacrylate (PEGDA) crosslinker (Extralink), resulting in a hydrogel.
2D & 3D
Embryonic stem cells (ESCs), Spheroids
A-549 (RRID:CVCL_0023)
HMC3 (RRID:CVCL_II76)
UP-007 (RRID:CVCL_AP56)
HMC3 (RRID:CVCL_II76)
UP-007 (RRID:CVCL_AP56)
Hepatocytes & HCT 116 (RRID:CVCL_0291) & Mesenchymal stem/stromal cells (MSCs)
HMC3 (RRID:CVCL_II76) & UP-007 (RRID:CVCL_AP56)
HMC3 (RRID:CVCL_II76) & UP-007 (RRID:CVCL_AP56)
Yes
No
Yes
Specified
G’ = 140 Pa
Yes
Michael addition reaction. Peptides with a N-terminal cysteine can be covalently linked to the hydrogel. The heparin forms ionic bonds with proteins, allowing controlled release of growth factors.
Unspecified
Yes
Enzymatic degradation (Collagenase/Hyaluronidase)
Unspecified
Unspecified
Yes