PeptiGel Alpha 1

Cell Guidance Systems
https://www.cellgs.com/products/peptigelandreg-alpha-1and8482.html
Synthetic

Self-assembled peptide hydrogel (neutrally charged)

By adding cell culture media. The ionic salts present in the media screen the charges present on the peptide fibres which causes them to aggregate and induce gelation at room temperature.

2D & 3D
Induced pluripotent stem cells (iPSCs), Spheroids, Other
A2780 (RRID:CVCL_0134)
EpH4 (RRID:CVCL_0073)
HEK293 (RRID:CVCL_0045)
MCF-10A (RRID:CVCL_0598)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
NIH 3T3 (RRID:CVCL_0594)
SK-OV-3 (RRID:CVCL_0532)
Yes
Yes
Yes
Specified

Stiffness = 3−5 kPa

Yes

Unspecified

Unspecified
Unspecified
Unspecified
Unspecified
Yes

Protocol – 2D cell culture
Protocol – 3D cell culture
Protocol – How to coat a well plate

Protocol – PicoGreen cell viability assay for PeptiGel cultures
Protocol – Sample preparation for protein extraction from PeptiGel cultures
Protocol – Sample preparation for RNA extraction from PeptiGel cultures
Protocol – How to process PeptiGel cultures for cell staining