PeptiGel Alpha 2

Cell Guidance Systems
https://www.cellgs.com/products/peptigelandreg-alpha-2and8482.html
Synthetic

Self-assembled peptide hydrogel (positively charged)

By adding cell culture media. The ionic salts present in the media screen the charges present on the peptide fibres which causes them to aggregate and induce gelation at room temperature.

2D & 3D
Organoids
A2780 (RRID:CVCL_0134)
HEK293 (RRID:CVCL_0045)
Hep-G2 (RRID:CVCL_0027)
LNCaP (RRID:CVCL_0395)
NCTC clone 929 (RRID:CVCL_0462)
NIH 3T3 (RRID:CVCL_0594)
PC-3 (RRID:CVCL_0035)
PNT2 (RRID:CVCL_2164)
SK-OV-3 (RRID:CVCL_0532)
SUIT-2 (RRID:CVCL_3172)
Adipose-derived stem/stromal cells (ASCs) & Dorsal root ganglion neurons
Yes
Yes
Yes
Specified

Stiffness = 6−8 kPa

Yes

Unspecified

Unspecified
Unspecified
Unspecified
Unspecified
Unspecified
Yes

Protocol – 2D cell culture
Protocol – 3D cell culture
Protocol – How to coat a well plate

Protocol – PicoGreen cell viability assay for PeptiGel cultures
Protocol – Sample preparation for protein extraction from PeptiGel cultures
Protocol – Sample preparation for RNA extraction from PeptiGel cultures
Protocol – How to process PeptiGel cultures for cell staining
Protocol – How to extract organoids from PeptiGel cultures