PeptiMatrix
Synthetic
Self-assembling peptide hydrogel (12.5−17.5 mg mL−1)
Gelation is induced by pH neutralisation on addition of cell culture medium. The gelation mechanism is pH and temperature dependent.
2D & 3D
Embryonic stem cells (ESCs), Spheroids
Hep-G2 (RRID:CVCL_0027)
HepaRG (RRID:CVCL_9720)
HMFU19
MCF-7 (RRID:CVCL_0031)
MOLM-13 (RRID:CVCL_2119)
NIH 3T3 (RRID:CVCL_0594)
OCI-AML-3 (RRID:CVCL_1844)
THP-1 (RRID:CVCL_0006)
U-87MG ATCC (RRID:CVCL_0022)
HepaRG (RRID:CVCL_9720)
HMFU19
MCF-7 (RRID:CVCL_0031)
MOLM-13 (RRID:CVCL_2119)
NIH 3T3 (RRID:CVCL_0594)
OCI-AML-3 (RRID:CVCL_1844)
THP-1 (RRID:CVCL_0006)
U-87MG ATCC (RRID:CVCL_0022)
Yes
Yes
Yes
Specified
G’ = 400−1200 Pa
Yes
Bioactive motifs are synthesised as an extension of the gelator peptide, and therefore, covalently immobilised to the hydrogel.
Unspecified
Yes
The hydrogel transitions into the liquid phase upon diluting the hydrogel with PBS or cell culture medium, which lowers the peptide concentration below the critical gelation concentration.
Yes
No
Yes
RGD