Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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Synthegel Spheroid Matrix Kit

Corning
https://ecatalog.corning.com/life-sciences/b2c/US/en/Surfaces/Extracellular-Matrices-ECMs/Corning%C2%AE-Synthegel%C2%AE-3D-Matrix-Kits/p/354789
Synthetic

Peptide hydrogel (2% w/v)

By mixing the peptide solution with the cross-linking reagent (Synthegel X-Link solution)

3D
Spheroids
4T1 (RRID:CVCL_0125)
A-549 (RRID:CVCL_0023)
FHC (RRID:CVCL_3688)
HeLa (RRID:CVCL_0030)
Hep-G2 (RRID:CVCL_0027)
LET1 (RRID:CVCL_A7VS)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
PANC-1 (RRID:CVCL_0480)
PC-3 (RRID:CVCL_0035)
Yes
Unspecified
Yes
Unspecified
Unspecified
Yes

By mechanical disruption

Unspecified
Unspecified
Yes

Protocol – Synthegel Spheroid Matrix Kit

Protocol – Cell recovery and immunostaining

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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