TrueGel3D Hydrogel Kit 3 (TRUE3) | Basement Membrane Extract (BME)-free Database

Commercial source

Merck, Sigma-Aldrich

URL

https://www.sigmaaldrich.com/NL/en/product/sigma/true3

Product details

Origin

Natural

Type of material

Dextran-based hydrogel

Type of gelation/Coating

The (thiol-reactive) maleimide-functionalised dextran polymer is crosslinked by the thiol-functionalised cyclodextrin (CD) cell-degradable crosslinker.

Type of in vitro culture

Type of application

Spheroids, Other

Cell type

Cell lines

MCF-7 (RRID:CVCL_0031)

Primary cells

Blood

Dendritic cells

Lymphocytes

Neutrophils

Skin

Dermal fibroblasts

Co-culture

Dermal fibroblasts & MCF-7 (RRID:CVCL_0031)

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Unspecified

Possibility to conjugate biomolecules

Yes

Conjugation method

Thiol-mediated conjugation. Peptides with a N- or C-terminal cysteine can be covalently linked to the hydrogel.

Application specifications

Cell retrieval

Yes

TrueGel3D Enzymatic Cell Recovery Solution (enzymatic)

Dome formation

Unspecified

Imaging

Autofluorescent

Unspecified

Transparent

Yes

Instructions

Product instructions

Downstream analysis

Protocol – Cell recovery
Protocol – How to customize the TrueGel3D™ hydrogel

Keywords

Invasion assay, Migration assay