TheWell Bioscience
Synthetic
Polysaccharide-based hydrogel
Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)
2D & 3D
Spheroids, Other
94T778 (RRID:CVCL_U613)
B35 (RRID:CVCL_1951)
BL-5 (RRID:CVCL_M563)
HBEC-KRAS
HCT 116 (RRID:CVCL_0291)
HeLa (RRID:CVCL_0030)
KHOS/NP (RRID:CVCL_2546)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
Nthy-ori 3-1 (RRID:CVCL_2659)
OP9/G (RRID:CVCL_B217)
PANC-1 (RRID:CVCL_0480)
Priess (RRID:CVCL_E813)
SNB-75 (RRID:CVCL_1706)
T-47D (RRID:CVCL_0553)
U2OS (RRID:CVCL_0042)
B35 (RRID:CVCL_1951)
BL-5 (RRID:CVCL_M563)
HBEC-KRAS
HCT 116 (RRID:CVCL_0291)
HeLa (RRID:CVCL_0030)
KHOS/NP (RRID:CVCL_2546)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
Nthy-ori 3-1 (RRID:CVCL_2659)
OP9/G (RRID:CVCL_B217)
PANC-1 (RRID:CVCL_0480)
Priess (RRID:CVCL_E813)
SNB-75 (RRID:CVCL_1706)
T-47D (RRID:CVCL_0553)
U2OS (RRID:CVCL_0042)
Yes
Yes
Yes
Specified
G’ = 10−4000 Pa
Yes
Unspecified
Yes
VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)
No
No
Yes
Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit
Protocol – Cell invasion assay
Invasion assay