VitroGel COL High Concentration

TheWell Bioscience
https://www.thewellbio.com/product/vitrogel-col/
Synthetic

Polysaccharide-based hydrogel

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)

2D & 3D
Embryonic stem cells (ESCs)
A2058 (RRID:CVCL_1059)
B16-F10 (RRID:CVCL_0159)
C2C12 (RRID:CVCL_0188)
HCEC-B4G12 (RRID:CVCL_2065)
HEK293 (RRID:CVCL_0045)
Hep 3B2.1-7 (RRID:CVCL_0326)
Hep-G2 (RRID:CVCL_0027)
HFL1 (RRID:CVCL_0298)
HT-1080 (RRID:CVCL_0317)
K1735 (RRID:CVCL_F828)
KM155C25 (RRID:CVCL_6B24)
LNCaP (RRID:CVCL_0395)
MCF-10A (RRID:CVCL_0598)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
MRC-5 (RRID:CVCL_0440)
NIH 3T3 (RRID:CVCL_0594)
OP9/G (RRID:CVCL_B217)
OVCA429 (RRID:CVCL_3936)
PANC-1 (RRID:CVCL_0480)
PC-3 (RRID:CVCL_0035)
PC12 (RRID:CVCL_0481)
RuGli (RRID:CVCL_JZ16)
SK-MEL-28 (RRID:CVCL_0526)
SNB-75 (RRID:CVCL_1706)
T-47D (RRID:CVCL_0553)
U-373MG ATCC (RRID:CVCL_2219)
U-87MG ATCC (RRID:CVCL_0022)
WM239A (RRID:CVCL_6795)
3T3-J2 (RRID:CVCL_W667) & Hep-G2 (RRID:CVCL_0027)
Dermal fibroblasts & Epidermal keratinocytes
Yes
Yes
Yes
Specified

G’ = 10−4000 Pa

Yes

Unspecified

Unspecified
Yes

VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)

No
Yes

Protocol – VitroGel COL High Concentration

Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit
Protocol – Cell invasion assay

Collagen, Invasion assay