TheWell Bioscience
Synthetic
Polysaccharide-based hydrogel
Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)
2D & 3D
Induced pluripotent stem cells (iPSCs)
A2058 (RRID:CVCL_1059)
K1735 (RRID:CVCL_F828)
LL29 (AnHa) (RRID:CVCL_2576)
LRM55
MCF-7 (RRID:CVCL_0031)
MIN6 (RRID:CVCL_0431)
PC-3 (RRID:CVCL_0035)
PC12 (RRID:CVCL_0481)
SK-MEL-28 (RRID:CVCL_0526)
SNB-75 (RRID:CVCL_1706)
K1735 (RRID:CVCL_F828)
LL29 (AnHa) (RRID:CVCL_2576)
LRM55
MCF-7 (RRID:CVCL_0031)
MIN6 (RRID:CVCL_0431)
PC-3 (RRID:CVCL_0035)
PC12 (RRID:CVCL_0481)
SK-MEL-28 (RRID:CVCL_0526)
SNB-75 (RRID:CVCL_1706)
Bone marrow-derived mesenchymal stem/stromal cells (BMSCs) & Outgrowth endothelial cells
Yes
Yes
Yes
Specified
G’ = 10−4000 Pa
Yes
Unspecified
Unspecified
Yes
VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)
No
Yes
Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit
Protocol – Cell invasion assay
Invasion assay, Laminin