VitroGel NEURON | Basement Membrane Extract (BME)-free Database

Commercial source

TheWell Bioscience

URL

https://www.thewellbio.com/product/neuron-culture-vitrogel/

Product details

Origin

Synthetic

Type of material

Polysaccharide-based hydrogel

Type of gelation/Coating

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca²⁺ or Na⁺)

Type of in vitro culture

2D & 3D

Type of application

Induced pluripotent stem cells (iPSCs), Organoids, Spheroids, Other

Cell type

Cell lines

B35 (RRID:CVCL_1951)
SH-SY5Y (RRID:CVCL_0019)

Stem cells

Adult stem cells

Neural stem cells (NSCs)

Induced pluripotent stem cells (iPSCs)

General

Pluripotent stem cell (PSC)-derived cells

Induced pluripotent stem cell (iPSC)-derived cells

Neural stem cells (NSCs)

Organoids

Induced pluripotent stem cell (iPSC)-derived

Midbrain organoids

Co-culture

Neural stem cells (NSCs) & Oligodendrocytes

Product specifications

Commercially available

Yes

Animal-free

Yes

Chemically defined

Yes

Mechanical properties

Specified

G’ = 100−1000 Pa

Possibility to conjugate biomolecules

Yes

Conjugation method

Covalent conjugation

Application specifications

Support expansion (Organoids)

Unspecified

Reference

Support differentiation (Organoids)

Unspecified

Reference

Support maintenance pluripotency (PSCs)

Reference

Cell retrieval

Yes

VitroGel Organoid Recovery Solution (enzyme-free)

Dome formation

Yes

Imaging

Autofluorescent

Transparent

Yes

Instructions

Product instructions

Protocol – VitroGel NEURON

Downstream analysis

Protocol – VitroGel Organoid Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cyto3D Live-Dead Assay Kit

Keywords

Migration assay