Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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VitroGel ORGANOID-2

TheWell Bioscience
https://www.thewellbio.com/product/3d-organoid-culture-hydrogel/
Synthetic

Polysaccharide-based hydrogel

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)

2D & 3D
Organoids
Yes
Yes
Yes
Unspecified
Yes

Unspecified

Unspecified
Unspecified
Yes

VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)

Yes
No
Yes

Protocol – VitroGel Organoid

Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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