Basement Membrane Extract (BME)-free Database

Basement Membrane Extract (BME)-free Database

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VitroGel ORGANOID Discovery Kit

TheWell Bioscience
https://www.thewellbio.com/product/3d-organoid-culture-hydrogel/
Synthetic

Polysaccharide-based hydrogel. Discovery kit consists of VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3 and VitroGel ORGANOID-4.

Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)

2D & 3D
Embryonic stem cells (ESCs), Induced pluripotent stem cells (iPSCs), Organoids
4T1 (RRID:CVCL_0125)
94T778 (RRID:CVCL_U613)
A-549 (RRID:CVCL_0023)
A2058 (RRID:CVCL_1059)
AU565 (RRID:CVCL_1074)
B16-F10 (RRID:CVCL_0159)
Beta-TC-3 (RRID:CVCL_0172)
BL-5 (RRID:CVCL_M563)
C2C12 (RRID:CVCL_0188)
DLD-1 (RRID:CVCL_0248)
DU145 (RRID:CVCL_0105)
EO771 (RRID:CVCL_GR23)
Fuji (RRID:CVCL_D880)
HBEC-KRAS
HCEC-B4G12 (RRID:CVCL_2065)
HCT 116 (RRID:CVCL_0291)
HCT 8 (RRID:CVCL_2478)
HEK293 (RRID:CVCL_0045)
HeLa (RRID:CVCL_0030)
Hep-G2 (RRID:CVCL_0027)
HFL1 (RRID:CVCL_0298)
HT-1080 (RRID:CVCL_0317)
INS-1 (RRID:CVCL_0352)
K1735 (RRID:CVCL_F828)
KHOS/NP (RRID:CVCL_2546)
KM155C25 (RRID:CVCL_6B24)
LL/2 (LLC1) (RRID:CVCL_4358)
LNCaP (RRID:CVCL_0395)
LRM55
MCF-10A (RRID:CVCL_0598)
MCF-12A (RRID:CVCL_3744)
MCF-7 (RRID:CVCL_0031)
MDA-MB-231 (RRID:CVCL_0062)
MDCK (RRID:CVCL_0422)
MIN6 (RRID:CVCL_0431)
MRC-5 (RRID:CVCL_0440)
NIH 3T3 (RRID:CVCL_0594)
Nthy-ori 3-1 (RRID:CVCL_2659)
OSA 1777 (RRID:CVCL_ZV94)
OV-MZ-6 (RRID:CVCL_4005)
OVCA429 (RRID:CVCL_3936)
OVCAR-3 (RRID:CVCL_0465)
PANC-1 (RRID:CVCL_0480)
PC-3 (RRID:CVCL_0035)
Priess (RRID:CVCL_E813)
RLE-6TN (RRID:CVCL_4693)
RuGli (RRID:CVCL_JZ16)
SF268 (RRID:CVCL_1689)
SF295 (RRID:CVCL_1690)
SK-MEL-28 (RRID:CVCL_0526)
SK-OV-3 (RRID:CVCL_0532)
SNB-75 (RRID:CVCL_1706)
SYO-1 (RRID:CVCL_7146)
T-47D (RRID:CVCL_0553)
U-251MG (RRID:CVCL_0021)
U-373MG ATCC (RRID:CVCL_2219)
U-87MG ATCC (RRID:CVCL_0022)
U2OS (RRID:CVCL_0042)
WM239A (RRID:CVCL_6795)
Gastric organoids & Dendritic cells
Yes
Yes
Yes
Unspecified
Yes

Unspecified

Unspecified
Unspecified
Unspecified
Yes

VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)

Yes
No
Yes

Protocol – VitroGel Organoid

Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit

Contact us: 3RCU@uu.nl
3Rs Centre Utrecht  
Utrecht University
Bolognalaan 50 3584 CJ 
Utrecht, The Netherlands

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The development of the Basement Membrane Extract (BME)-free Database has been supported by Proefdiervrij

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