TheWell Bioscience
Synthetic
Polysaccharide-based hydrogel
Mixing the hydrogel with cell culture medium at room temperature (interaction with ionic molecules like Ca2+ or Na+)
2D & 3D
Embryonic stem cells (ESCs), Induced pluripotent stem cells (iPSCs), Spheroids
Yes
Yes
Yes
Specified
G’ = 100−1000 Pa
Yes
Unspecified
Yes
Yes
VitroGel Cell Recovery Solution or VitroGel Organoid Recovery Solution (enzyme-free)
No
No
Yes
Protocol – VitroGel Organoid Recovery Solution
Protocol – Cell recovery from gel using VitroGel Cell Recovery Solution
Protocol – Sample preparation for histological analysis
Protocol – Immunofluorescence staining
Protocol – Cell proliferation assay
Protocol – Cell lysis for downstream analysis
Protocol – Cell fluorescent staining in gel for nuclei and actin filament
Protocol – Cyto3D Live-Dead Assay Kit